|
KMID : 0380020090240020170
|
|
Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 2 p.170 ~ p.176
|
|
|
Biochemical Properties of Seed Lectin from Korean Soybean Cultivars Developed for Soy Source
|
|
Wang Yushan
Roh Kwang-Soo
|
|
|
Abstract
|
|
|
|
Lectin was finally isolated on Sephadex G-100 from Korean soybean cultivars developed for soy source and investigated its some biochemical properties. Native PAGE pattern of this lectin revealed a molecular weight of 108 kDa as tetramer. The molecular weight of this lectin isolated as double protein band by SDS-PAGE was calculated to be 32 and 22 kDa from the relative mobilities compared with those of the standard proteins. Among the tested red blood cell, the isolated lectin agglutinated rabbit red blood cell treated with trypsin, but did not agglutinated human red blood cells (A, B, AB, O), rat, and untreated rabbit red blood cell. The optimal temperature and thermal stability of isolated lectin was at 20- and 10-, respectively. This lectin was stable at 7.2, and showed complete loss in its activity below pH 6.2 and above pH 8.0.
|
|
|
KEYWORD
|
|
|
Biochemical property, hemagglutination activity, Korean soybean cultivars, lectin
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|